Brown ground: a soil carbon analogue for the green world hypothesis? The American Naturalist, 167(5), 619–627.Īmann, R. Characterization of urease and carbonic anhydrase producing bacteria and their role in calcite precipitation. PhD Thesis Cochin university of Science and Technology.Īchal, V., & Pan, X.
Metagenomics of marine and mangrove sediments: phylogenetic diversity and characterization of amylase obtained by functional screening. altitudinis M8 strain could be effectively employed in the process of carbon dioxide sequestration.Ībilash, P. The carbonic anhydrase-mediated calcium precipitation by B.
MICROCOSM BAND FREE
There was a reduction of 42.22 ± 0.23% of free calcium in the medium through calcite precipitation. To further understand the bio-mineralization ability of this microbial isolate, calcium precipitation assay was conducted. Hence, the application of enzyme was found to be more effective in removing CO 2 when compared to bacterial inoculum. altitudinis M8 strain could reduce CO 2 by 75 ± 0.12% in microcosm composed of sterilized soil with bacteria (SSB) and by 97 ± 0.34% in microcosm with sterile soil with enzyme (SSE). Soil microcosm was established to study carbonic anhydrase production and CO 2 sequestration ability of B. The potential isolate which higher CA production, M8 was identified as Bacillus altitudinis based on 16S rDNA sequencing. The enzyme activity increased by 1.6-fold upon precipitation by acetone (80%).
The specific activity of the partially purified CA enzyme from M3 and M8 were 44 U mg −1 and 76 U mg −1 respectively. Based on the secondary screening, M3 and M8 were identified as the most potential for carbonic anhydrase production. Fifteen bacterial colonies (M1–M15) isolated were screened for their carbonic anhydrase enzyme production potential based on p-nitro phenol acetate assay. In this study, we attempt to prospect potential bacterial isolates from mangrove sediments of Mangalavanam, Kerala, India, with positive carbonic anhydrase (CA) activity to sequester carbon dioxide by calcium precipitation process.
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